Morphological transformation induced by glass fibers in BALB/c-3T3 cells.
Gao-G; Whong-Z; Jones-WG; Wallace-WE; Ong-T
Teratog, Carcinog, Mutagen 1995 Jul; 15(2):63-71
To investigate the carcinogenicity of man made glass fibers, a study was undertaken to determine the ability of these fibers to transform BALB/c-3T3 cells and to elucidate the neoplastic potential of these cells after transformation. To determine transformation, cultures of BALB/c-3T3 cells were exposed to five concentrations each of three glass fiber varieties (ISL, AAA-10, and JM-10) for 72 hours. The neoplastic potential of transformed cells was determined via soft agar cloning and gene transfection analysis. Relative colony forming efficiencies (RCE) were used to determine glass fiber cytotoxicity. All fiber types were shown to be cytotoxic, with JM- 100 and AAA-10 proving to be more cytotoxic than ISL at higher concentrations (greater than 10 micrograms/cubic meter). All glass fiber types produced concentration dependent transformation of BALB/c-3T3 cells. From this study the authors conclude that glass fibers are capable of transforming BALB/c-3T3 cells, and that such transformed cells exhibit characteristics suggestive of neoplastic potential.
NIOSH-Author; Fibrous-glass; In-vitro-study; Carcinogens; Cell-transformation; Neoplastic-transformation; Cell-cultures; Mammalian-cells; Cytotoxic-effects;
Author Keywords: glass fiber; transformation; BALB/c-3T3; transfection; anchorage independence
W.Z. Whong, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505-2888
Teratogenesis, Carcinogenesis, and Mutagenesis