Biological monitoring of exposure to polycyclic aromatic hydrocarbons.
Santella RM; Zhang YJ; Young TL; Lil Y; Toor M; Leel BM; Stefanidis M; Warburton D; DeLeo V; Perera FP
Anticarcinogenesis and radiation protection 2. Nygaard OF, Upton AC, eds. New York: Plenum Press, 1991 Jan; :155-169
The sensitivities and limitations of various methods for detection of polycyclic aromatic hydrocarbon (PAH) DNA adducts in human studies were discussed. Enzyme linked immunosorbent assays (ELISA) using antibodies to benzo(a)pyrene-diol-epoxide-DNA (BPDE-I-DNA) as a measure of benzo(a)pyrene (50328) (BP) exposure were found to be less specific than previously thought. A comparison of PAH-DNA adducts in white blood cell (WBC) DNA from Finnish foundry and Polish coke oven workers showed good correlation between adduct levels measured by ELISA and phosphorus-32 (P32) postlabeling as well as for level of worker exposure. Use of the monoclonal antibodies in measurement of protein adducts on albumin in roofers detected no significant differences in adduct levels. Screening for PAH diol epoxide DNA adducts in sera from Finnish foundry workers and roofers did not detect significant differences from control adduct levels, while use of WBC DNA adduct immunoassays showed considerable increases in exposed workers over control levels. Blood samples from 22 psoriasis patients treated with topical application of coal-tar and five controls were analyzed for sister chromatid exchange (SCE) and micronuclei (MN) in peripheral lymphocytes, ELISA and (32P) postlabeling assays for WBC PAH DNA adducts, and serum antibodies to BPDE-I-DNA adducts. ELISA of WBC detected PAH diol epoxide DNA adducts in 13 of 22 patients and in one of five controls. Postlabeling and SCE levels were also higher for treated patients. Epidermis biopsies stained with polyclonal adduct antibody had specific staining in the stratum spinosum and granulosum of treated patients which was absent in controls. Postlabeling of DNA from skin biopsies detected multiple DNA adducts. The authors conclude that immunoassays in combination with other detection methods are sufficiently sensitive to monitor human environmental PAH exposure.
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