Functional alteration of mammalian cells by direct high frequency electromagnetic field interactions.
Cleary-SF; Liu-M; Cao-G
Charge and Field Effects in Biosystems, Vol. II 1989 Jan; :211-221
The biological effects of high frequency electromagnetic (microwave) radiation on in-vitro mammalian cell systems were discussed. The rationale for using in-vitro systems to investigate the bioeffects of microwave radiation was considered. The results of studies investigating direct nonthermal effects of microwave radiation in erythrocyte, lymphocyte, and glioma cell cultures were discussed. Microwave irradiation of erythrocyte cultures has been shown to exert direct effects on specific membrane molecules or macromolecular complexes, rather than more general interactions resulting in global changes in membrane properties. Continuous wave (CW) 100 megahertz (MHz) radiation inducing an electric field of 400 volts per meter (V/m) caused hemolysis, whereas at 10MHz an electric field strength of greater than 900V/m was required to cause the same hemolytic effect. Studies of the effects of microwave radiation on human peripheral lymphocytes maintained under isothermal conditions have shown that their proliferative activity, measured by changes in uptake of tritiated thymidine, is altered. For example, exposure of phytohemagglutinin stimulated lymphocytes to 2.45 gigahertz (GHz) CW microwave radiation at a specific absorption rate (SAR) of 50 watts per kilogram (W/kg), equivalent to a 145V/m electric field, suppressed cellular proliferation. Exposure at SARs of 25 or 39.5W/kg, equivalent to 72 or 114.5V/m electric fields, however, significantly increased cell proliferation. Similar SAR dependent biphasic responses have been seen in glioma cells exposed to 27MHz or 2.45GHZ microwaves under isothermal conditions. The authors conclude that the biphasic dose dependent changes in proliferative cell activity seen in in-vitro cell cultures exposed to microwave radiation might explain the unexplained variability seen in in-vivo studies.
NIOSH-Grant; Reproductive-system-disorders; Microwave-radiation; Mammalian-cells; Electromagnetic-radiation; Electrical-fields; In-vitro-studies; Cell-division; Dose-response
Physiology and Biophysics Medical College of Virginia Box 694, Mcv Station Richmond, VA 23298
Allen-M; Cleary-S; Hawkridge-F
Charge and Field Effects in Biosystems, Vol. II
Virginia Commonwealth University, Richmond, Virginia