Detection of DNA adducts in skin biopsies of coal tar-treated psoriasis patients: immunofluorescence and 32P postlabeling.
Zhang-YJ; Li-Y; DeLeo-VA; Santella-RM
Skin Pharmacol 1990 Apr; 3(3):171-179
Skin biopsies were obtained from patients treated for psoriasis and from controls in order to study the formation of covalent DNA adducts resulting from treatment with crude coal-tar (8007452) sometimes followed by ultraviolet (UV) irradiation. DNA adducts were analyzed by phosphorus-32 postlabeling and indirect immunohistochemical staining with antibodies against diol epoxide adducts of DNA. Indirect immunofluorescence staining indicated specific nuclear staining in the stratum spinosum, granulosum and basale of the epidermis. Staining was also scattered throughout the dermis sometimes localized to fibroblasts and vessels. No staining was noted in control samples. Isolation of DNA from patients provided 3.9 to 7.5 micrograms of DNA for postlabeling assay. Multiple adduct patterns were similar in all samples. Total adduct levels ranged from 0.18 to 9.4 adducts per 10(8) nucleotides. Adducts in DNA from control samples were below the limit of detection which was 0.1 per 10(8) nucleotides. The authors conclude that topical crude coal-tar preparation application produced detectable DNA adduct levels in the skin of psoriasis patients.
NIOSH-Publication; NIOSH-Grant; Dermatitis; DNA-damage; Skin-exposure; DNA-adducts; Medical-treatment; Skin-disorders; Humans
Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032
Columbia University New York, New York, New York