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The role of antioxidants in skin immune reactions: the use of flow cytometry to determine alterations in Ia-positive epidermal cells in allergic contact dermatitis.
Rheins-LA; Moravec-RA; Nordlung-ML; Trinkle-LS; Nordlund-JJ
J Soc Cosmet Chem 1989 Mar; 40():101-107
In-vivo and in-vitro studies were conducted to examine the density of the Class-II major histocompatibility Ia antigen on epidermal immune cells previously treated with various antioxidants or arachidonic-acid (AA). Male DBA/2d-mice and C57BL/6b-mice were used in the study. DBA/2-mice were treated topically for 5 days with 20% monobenzyl-ether-hydroquinone (103162) (MBEH), 0.05 or 1.0% AA, or diluent (control) to dorsal pinnal epidermal surfaces. On day six, the ears were surgically excised and the cells subjected to fluorescent activated cell sorting. In the control group, 1.4% of the cells were Ia+ Langerhans cells compared to 3.3% in the MBEH treated group. Normal Langerhans cell density, cells/square millimeter, made up about 2% of the total epidermal cell density in this strain. The mean fluorescence intensity increased from 69.51% to 72.99%. In in-vitro studies unenriched epidermal cell suspensions were exposed to concentrations of arachidonic-acid alone or with butylated-hydroxyanisole (25013165) (BHA), butylated- hydroxytoluene (128370) (BHT) and the control diluents. Cell preparations were harvested after 48 hours and subjected to fluorescent activated cell sorting (FACS) analysis. An apparent biphasic effect was noted following exposure to low or high doses of AA with or without the BHA and BHT exposures. A significant decrease was noted in the quantity of Ia+ expression from unenriched epidermal cell suspensions following exposure to a large dose of AA. Addition of indomethacin to the cell culture containing high AA doses restored Ia antigenic expression to control levels. The authors conclude that the studies support the effective use of FACS as a sensitive and precise tool to measure small yet significant identifiable changes in epidermal immune molecules residing in the epidermis.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Skin-exposure; Laboratory-animals; Cytotoxic-effects; Contact-dermatitis
Dermatology University of Cincinnati 231 Bethesda Avenue Cincinnati, OH 45267
103-16-2; 25013-16-5; 128-37-0
Journal of the Society of Cosmetic Chemists
University of Cincinnati, Cincinnati, Ohio
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division