Pharmacologic characterization of wool dust extract in isolated guinea pig trachea.
Schachter-EN; Zuskin-E; Buck-MG; Maayani-S; Marom-Z; Goswami-S; Rienzi-N
Environ Res 1995 May; 69(2):90-95
The airway effects of wool dust were examined in guinea-pig tracheal rings and compared with previous results obtained for cotton bract extract (CBE). Wool dust was collected from carding machines at a mill in Croatia. Wool dust extract (WDE) was prepared by incubating wool with sterile distilled water in a weight to volume ratio of 1 gram wool dust to 10 milliliters sterile water for 24 hours. Tracheal tissue came from male albino-Hartley-guinea-pigs. Four segments each 4 to 6 millimeters wide, were cut from a single trachea and suspended between two L-shaped stainless steel hooks in an organ chamber. Dose response curves to WDE were measured in 30 guinea-pig tracheal rings obtained from different animals and expressed as a percentage of maximal carbachol contraction. WDE produced bronchoconstriction defined by a dose response curve similar to CBE. The maximal muscle tension for WDE was 57.9%, compared with 42.3% for CBE. Pyrilamine partially blocked the constricting effect of WDE at the highest WDE doses. Verapamil and trimethoxybenzoic-acid completely blocked the response to WDE. In contrast, CBE induced bronchoconstriction was significantly inhibited by a number of chemical agents, including atropine, indomethacin, and pyrilamine. Endotoxin was not present in the WDE. The authors conclude that WDE may have clinical effects related to non immunoglobulin-E mechanisms, which could have therapeutic implications for respiratory symptoms in wool textile workers.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Laboratory-animals; Textile-mills; Respiratory-hypersensitivity; In-vitro-study; Wools
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