Antitransforming activity of chlorophyllin against selected carcinogens and complex mixtures.
Wu ZL; Chen JK; Ong T; Brockman HE; Whong Z
Teratog, Carcinog, Mutagen 1994 Jan; 14(2):75-81
The in-vitro antitransforming activity of chlorophyllin against various carcinogens and carcinogenic mixtures was examined. Chlorophyllin at concentrations of 0.15mg/ml or less was found not to be cytotoxic to cells cultured at 2x10(4) cells/3 milliliters (ml). BALB/3T3 cells at 2x10(4)cells/3ml were incubated with 0.15mg/ml chlorophyllin in the presence or absence of 0.2 microgram per milliliter (microg/ml) benzo(a)pyrene (50328) (BaP), 2.5microg/ml N-methyl-N'-nitro-N-nitrosoguanidine (70257) (MNNG), 0.1microg/ml aflatoxin-B1 (1162658) (AFB1), 5microg/ml 3- methylcholanthrene (56495) (3MC), 0.25microg/ml 7,12- dimethylbenz(a)anthracene (57976) (DMBA), 4.16mg/ml nitrosated coal dust, 1.76mg/ml nitrosated chewing tobacco, or 1.4mg/ml acidified tobacco snuff. The effects of chlorophyllin on the transforming activity of the carcinogens and mixtures were assessed by scoring the cells for transformation foci. Chlorophyllin significantly decreased the number of transformation foci induced by BaP, MNNG, AFB1, 3MC, DMBA, and the coal dust, chewing tobacco, and snuff. The authors conclude that chlorophyllin can inhibit the in-vitro transforming activity of known carcinogens and carcinogenic mixtures. The exact mechanism of its antitransforming activity is unknown, although it may involve scavenging of oxygen free radicals and binding to active sites in mutagenic molecules.
NIOSH-Author; Natural-products; Cell-transformation; In-vitro-studies; Mammalian-cells; Coal-dust; Dose-response; Cytotoxic-effects;
Author Keywords: antitransformation; chlorophyllin; carcinogens; coal dust; smokeless tobacco
W.-Z. Whong, National Institute for Occupational Safety and Health, 944 Chestnut Ridge Road, Morgantown, WV 26505-2888
50-32-8; 70-25-7; 1162-65-8; 56-49-5; 57-97-6
Teratogenesis, Carcinogenesis, and Mutagenesis