Decreased resistance to Listeria Monocytogenes in mice following vanadate exposure: effects upon the function of macrophages.
Cohen-MD; Chen-M; Wei-C
Int J Immunopharmacol 1989 Apr; 11(3):285-292
The authors examined the effects of vanadate exposure on mouse peritoneal macrophages (PEM) with particular regard to the reduced resistance to Listeria-monocytogenes. Female B6C3F1-mice were dosed with 10 or 2.5mg/kg vanadate by intraperitoneal injection every 3 days for 6 weeks. Mice were then either administered a dose of viable Listeria or were sacrificed. A dose dependent increase in Listeria levels was noted in the spleen and liver at the end of the 6 week period. Mice treated with the 10mg/kg regimen showed detectable hepatic levels of Listeria as soon as 8 hours after injection; mice in the 2.5mg/kg dose group demonstrated Listeria by 12 hours. By 48 hours after the infection was induced, the 2.5 and 10mg/kg dose groups had levels of Listeria which were 200 and 1,300 fold higher than the initial dose, respectively. The total in-vitro phagocytic uptake of Listeria by peritoneal macrophages (PEM) was consistently decreased as a function of the infection period. Intracellular killing of Listeria was decreased even though the PEM from the vanadate treated and control mice were more bacteriostatic than bactericidal. The authors conclude that these findings offer additional information regarding the development of a mechanism to understand why workers exposed to vanadium (7440622) containing dusts are increasingly susceptible to bacterial respiratory diseases.
NIOSH-Publication; NIOSH-Grant; Grants-other; Bacterial-infections; Host-resistance; Laboratory-animals; Toxic-effects
Food Science & Human Nutrition University of Florida Gainesville, FL 32611 Gainesville, FL 32611
International Journal of Immunopharmacology
University of Florida, Gainesville, Florida