Specific binding of (3H)methylscopolamine to glass gibre gilters.
J Pharm Pharmacol 1987 Mar; 39(3):239-240
The binding of tritium labeled methylscopolamine (MS) to glass fiber filters was investigated. The amount of binding was dependent on the buffer used for the incubation. When atropine was present, total binding was decreased 50 to 60%. Specific binding was noted in the presence of scopolamine, methylscopolamine, methylatropine, carbachol, oxotremorine, and acetylcholine. Total binding increased with increasing concentrations of MS. Specific binding to glass fiber filters was saturable. Presoaking the glass fiber filters in bovine serum albumin solution decreased total binding 45 to 60%, and specific binding 70 to 85%. Pretreatment of the filters with poly-L- lysine reduced total binding to glass fiber filters by more than 80% and virtually abolished specific binding. The author suggests that these findings indicate the presence on glass fiber filters of a binding site for MS. The author urges caution when MS is used as a muscarinic ligand and that measures to reduce filter binding or alternative separation procedures should be considered.
NIOSH-Publication; NIOSH-Grant; Grants-other; Analytical-methods; Chemical-analysis; Analytical-chemistry; Filter-materials; Filtration;
Environmental Health University of Washington Dept of Environ Hlth, SC-34 Seattle, WA 98195
Other Occupational Concerns; Grants-other
Journal of Pharmacy and Pharmacology
University of Washington, Seattle, Washington