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Quantitative fluorescence image analysis of deoxyribonucleic acid ploidy in urine from normal children.
Moon-TD; Harmon-EP; Hurst-RE; Bass-RA; Colcolough-M; Hemstreet-GP III
J Urol 1991 Jun; 145(6):1236-1237
Urinary cells from children undergoing urological evaluation who were not known to have any malignant process were studied using quantitative fluorescence image analysis. With Hoechst-33258 the bladder cells demonstrated well defined nuclear fluorescence with practically no cytoplasmic fluorescence, similar to the pattern noted in adult bladder cells. Using acridine-orange, however, cells showed a wide variation in the amount of green cytoplasmic fluorescence. With acridine-orange, 8% of the specimens had more than two abnormal cells per 500 cells analyzed. Hoechst-33258 fluorochrome showed only one patient to have more than two cells per 500 analyzed with greater than 5C DNA. The authors suggest that the extremely frequent finding of acridine-orange positive cells compared with Hoechst-33258 positive cells indicates that a reactive epithelium and/or possible subclinical viral infection was operant in the children. The authors conclude that in light of these findings, the 8% false positive rate for asymptomatic men older than 50 years should be reconsidered. They note that only longitudinal studies will determine if these findings are representative of the development of a premalignant condition or merely the cumulative effects of aging and possible environmental exposure to toxicants.
NIOSH-Publication; NIOSH-Grant; Cancer; Analytical-methods; Risk-factors; Humans; DNA-damage
Urology University of Oklahoma Dept of Urology, PO Box 26901 Oklahoma City, OK 73190
Issue of Publication
Journal of Urology
University of Oklahoma Hlth Sciences Ctr, Oklahoma City, Oklahoma
Page last reviewed: April 12, 2019
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