Quantitative fluorescence image analysis of deoxyribonucleic acid ploidy in urine from normal children.
Moon-TD; Harmon-EP; Hurst-RE; Bass-RA; Colcolough-M; Hemstreet-GP III
J Urol 1991 Jun; 145(6):1236-1237
Urinary cells from children undergoing urological evaluation who were not known to have any malignant process were studied using quantitative fluorescence image analysis. With Hoechst-33258 the bladder cells demonstrated well defined nuclear fluorescence with practically no cytoplasmic fluorescence, similar to the pattern noted in adult bladder cells. Using acridine-orange, however, cells showed a wide variation in the amount of green cytoplasmic fluorescence. With acridine-orange, 8% of the specimens had more than two abnormal cells per 500 cells analyzed. Hoechst-33258 fluorochrome showed only one patient to have more than two cells per 500 analyzed with greater than 5C DNA. The authors suggest that the extremely frequent finding of acridine-orange positive cells compared with Hoechst-33258 positive cells indicates that a reactive epithelium and/or possible subclinical viral infection was operant in the children. The authors conclude that in light of these findings, the 8% false positive rate for asymptomatic men older than 50 years should be reconsidered. They note that only longitudinal studies will determine if these findings are representative of the development of a premalignant condition or merely the cumulative effects of aging and possible environmental exposure to toxicants.
NIOSH-Publication; NIOSH-Grant; Cancer; Analytical-methods; Risk-factors; Humans; DNA-damage
Urology University of Oklahoma Dept of Urology, PO Box 26901 Oklahoma City, OK 73190
Journal of Urology
University of Oklahoma Hlth Sciences Ctr, Oklahoma City, Oklahoma