Identification of a major human urinary metabolite of alachlor by LC- MS/MS.
Driskell-WJ; Hill-RH Jr.; Shealy-DB; Hull-RD; Hines-CJ
Bull Environ Contam Toxicol 1996 Jun; 56(6):853-859
Urinary metabolites of the herbicide alachlor (15972608) were identified in occupationally exposed subjects by liquid chromatography coupled to mass spectrometry. Urine samples were collected from four workers; daughter and parent scanning modes were then utilized to identify alachlor metabolites. On the basis of mass spectrometry and chromatographic behavior, the major metabolite was identified as alachlor-mercapturate. A method was then developed to measured this metabolite in urine. In the four urinary samples, alachlor-mercapturate levels ranged from 25 to 62% of the concentration of the alachlor hydrolysis product, 2,6-diethylaniline (579668) (DEA). No other alachlor metabolites were positively identified. The authors conclude that identification of alachlor- mercapturate allows for the development of novel methods to assess alachlor exposure and enable more effective biomonitoring; a method based on measurement of this metabolite should prove to be simpler, safer, and more specific than the previously used DEA method.
NIOSH-Author; Occupational-exposure; Herbicides; Biological-monitoring; Analytical-methods; Chromatographic-analysis; Urinalysis; Urine-chemistry; Mass-spectrometry
W. J. Driskell, National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA
Bulletin of Environmental Contamination and Toxicology