Determination of albumin and hemoglobin adducts in workers exposed to styrene and styrene oxide.
Yeowell-O'-Connell-K; Jin-Z; Rappaport-SM
Cancer Epidemiol Biomark Prev 1996 Mar; 5(3):205-215
A study of albumin and hemoglobin adduct formation in workers occupationally exposed to styrene (100425) and styrene-oxide (96093) was conducted. The study group consisted of 48 healthy workers employed at a factory manufacturing reinforced plastic boats during 1987 and 1988. Personal air exposures to styrene and styrene-oxide over a work shift were monitored for 1 year. Multiple blood samples were collected over a year. The hemoglobin and albumin were extracted and analyzed for cysteine and carboxylic acid adducts of styrene-oxide utilizing gas chromatography/mass spectrometry. Associations between the adduct concentrations and personal air styrene and styrene-oxide concentrations were analyzed by univariate or multivariate techniques. Personal air styrene and styrene-oxide exposures varied from 0.9 to 235mg/m3 and 13.4 to 525 micrograms per cubic meter (microg/m3), respectively. The mean exposure concentrations were 64.3mg/m3 and 159microg/m3. The mean styrene-oxide/hemoglobin adduct concentrations varied from 0.078 to 0.481 nanomole per gram protein (nmol/g). The mean styrene-oxide/albumin adduct concentrations varied from 0.290 to 1.80nmol/g. The styrene/hemoglobin concentrations showed no apparent association with styrene or styrene-oxide exposure. The concentrations of styrene/albumin adducts tended to increase linearly with increasing personal air styrene or styrene-oxide concentration. The associations were stronger for styrene-oxide than for styrene. The authors conclude that the stronger association between styrene-oxide/albumin adduct concentrations and styrene-oxide exposure than with styrene exposure suggests that styrene-oxide exposures, even though low, may be important for workers in the reinforced plastics industry.
Aromatic-hydrocarbons; Organic-solvents; Occupational-exposure; Chemical-binding; Hemoproteins; Biological-monitoring; Industrial-hygiene; Reinforced-plastics; Boat-manufacturing-industry
Cancer Epidemiology, Biomarkers & Prevention
University of California Berkeley, Berkeley, California