Sister-chromatid exchanges, glutathione s-transferase theta deletion and cytogenic sensitivity to diepoxybutane in lymphocytes from butadiene monomer production workers.
Kelsey-KT; Wiencke-JK; Ward-J; Bechtold-W; Fajen-J
Mutat Res 1995 Dec; 335(3):267-273
A study was conducted examining the genotoxicity of butadiene (106990) among occupationally exposed workers as well as a possible increased risk for individuals sensitive to diepoxybutane (298180) (DEB) and with deletion of the glutathione-S-transferase class theta gene (GSTT1). Baseline frequencies of lymphocytic sister chromatid exchanges (SCEs), DEB induced SCE frequencies, and GSTT1 deletion status were determined in 40 butadiene workers. The mean butadiene exposure levels during two analyses were found to be well below 1 part per million (ppm) and ranged from nondetectable to 8.53 ppm. Urinary butadiene metabolite 1,2-dihydroxy-4-(N-acetylcysteine-S-)- butane (M1) excretion did not correlate with butadiene exposure. Thirty four of the 40 workers studied were found to be resistant to SCE induction by DEB, whereas six were found to be sensitive. All DEB sensitive workers demonstrated deletion of GSTT1. No correlation was seen between the SCE frequency and exposure to butadiene or urinary M1 excretion. Only cigarette smoking and DEB sensitivity were significantly correlated with baseline SCE frequency.
NIOSH-Publication; NIOSH-Author; NIOSH-Grant; Grants-other; Butadienes; Chromosome-damage; Toxic-effects; Occupational-exposure; Metabolites; Genetic-factors; Genotoxic-effects; Cigarette-smoking
Environmental Health Harvard School of Public Hlth 665 Huntington Avenue Boston, MA 02115
Harvard University, Boston, Massachusetts