Glass-fiber-induced cell transformation and proto-oncogene activation in BALB/c-3T3 cells.
Ong-T-M; Gao-G; Cutler-DR; Stewart-JD; Jones-WG; Whong-Z
Sixth US-Finnish Joint Symposium on Occupational Health and Safety, People and Work, Proceedings of the Sixth FIOH-NIOSH Joint Symposium on Occupational Health and Safety, 8-10 August 1995, Espoo, Finland. People and Work - Research Reports 3. H. Nordman, J. Starck, A. Tossavainen, E. Viikari-Juntura, eds. Helsinki, Finland: Finnish Institute of Occupational Health; 1995 Aug; :50-54
The cell transforming activity of man made fibers was investigated using BALB/c-3T3 cells. The possible mechanism of glass fiber induced transformation in relation to proto oncogene activation was also examined by cell transformation assay. Three types of glass fibers were used: Owens Corning AAA-10, Manville code 100 (JM-100), and Owens Corning general building insulation (ISL). The size distribution of glass fibers was 0.18 and 2 micrometers (microm) for AAA-10, 0.2 and 3.5microm for JM-100, and 7.3 and 98microm for ISL. The samples were treated with and without each type of glass fibers for 72 hours. Concentration related transformation and cytotoxicity were observed in BALB/c-3T3 cells treated with the three different glass fibers. The concentrations which caused a significant increase in cell transformation were 1 to 38 micrograms/square centimeter (microg/cm2) for AAA-10, 10 to 38microg/cm2 for JM-100, and 38 to 160microg/cm2 for ISL. The transformation potencies among the three types of fiber were in the order: AAA-10 approximately equal to JM-100, both of which were greater than ISL. The authors conclude that glass fibers are able to transform mammalian cells in- vitro and the transforming potential depends on fiber size.
Airborne-fibers; Fibrous-dusts; Cytotoxic-effects; Cell-damage; Cell-cultures; Laboratory-animals; Fiberglass-industry
Nordman-H; Starck-J; Tossavainen-A; Viikari-Juntura-E
Sixth US-Finnish Joint Symposium on Occupational Health and Safety, People and Work, Proceedings of the Sixth FIOH-NIOSH Joint Symposium on Occupational Health and Safety, 8-10 August 1995, Espoo, Finland. People and Work - Research Reports 3