Silica-induced micronuclei and chromosomal aberrations in Chinese hamster lung (V79) and human lung (Hel 299) cells.
Nagalakshmi-R; Nath-J; Ong-T; Whong-Z
Mutat Res 1995 Aug; 335(1):27-33
The clastogenic activity of two different sized distributions of silica (14808607) dusts were investigated in cultured Chinese- hamster lung fibroblasts (V79 cells) and human embryonic lung (Hel- 299) cells using two cytogenetic assay systems. The two forms of silica used were Min-U-Sil-5 with an average particle size of 5 micrometers, and Min-U-Sil-10, mostly in the 10 micrometer size range. The micronucleus assay and chromosomal aberration analysis were conducted on cells cultured with silica at 40 to 320 micrograms/square centimeter. Results of a cell cycle kinetics analysis of Hel-299 cells in the presence of cytochalasin-B indicate that with increases in culture time, there was a decrease in the proportion of mononucleated cells. The frequency of micronucleated V79 cells showed a dose related increase which was tied to an increase in silica concentration. At the highest concentration, a 13 fold increase was noted in total micronucleated binucleated cells over controls. In the Hel 299 cells there was an increase in frequency of micronucleated binucleated cells in both Min-U-Sil-5 and Min-U-Sil-10 treated cultures. Results of the chromosomal aberration studies indicated that the aberrations had not significantly increased by exposure to either Min-U-Sil-5 or Min-U- Sil-10 in either V79 or Hel-299 cells over the controls. There were, however, a few gaps observed for both cell lines and both types of silica tested.
NIOSH-Author; In-vitro-studies; Mineral-dusts; Silica-dusts; Lung-cells; Genotoxic-effects; Cell-cultures; Respiratory-system-disorders; Chromosome-damage;
Author Keywords: Silica; Micronucleus; Chromosomal aberration; V79 cells; Hel 299 cells