A NIOSH site visit was conducted to evaluate the tuberculin skin testing (TST) program, assess laboratory practices, review the use of safety equipment, and determine the operational status of the ventilation system at a state public health laboratory. The visit was prompted by a request from the management of the facility. The facility had 13 laboratory employees, including three clerical staff employees, seven microbiologists, and three laboratory technicians. The preparation of Mycobacterium-tuberculosis (Mtb) specimens in the laboratory was described. Approximately 6,000 to 6,500 suspected Mtb infected specimens were processed annually by the laboratory, with less than 1% resulting in a positive identification of Mtb in the sample. Observations were made during sample processing for the assessment of work practices and procedures. A walk through survey of the facility and a visual evaluation of the ventilation system were performed. Air samples were collected. Recommendations were offered for issues such as safe work practices, containment equipment, personal protective equipment, and laboratory facilities.