Influence of particulates on occupational lung disease.
Warshawsky-D; Radike-M; Talaska-G; Miller-M
Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio 1994 Jun; :1-34
The ability of alveolar macrophages (AM) to phagocytize uncoated and benzo(a)pyrene (50328) (BaP) coated particulates was investigated. AM from male Syrian-golden-hamsters and Sprague-Dawley-rats were used. Particulates included crystalline-silica (14808607); precipitated, gelled, and fumed amorphous-silica (7631869); ferric- oxide (1309371); and aluminum-oxide (1344281). All forms of amorphous silica had smaller particle sizes and were cytotoxic, suggesting that more of the dose for the amorphous silicas entered the AM. Toxicity also appeared related to the surface properties, with those particles having more silanol groups being more cytotoxic. These same properties were important in the metabolic activation of BaP by AM. At a similar dose of BaP and particle, all of the BaP coated particles produced more BaP dihydrodiols than BaP alone. AM exposed to particles coated with BaP responded differently than either BaP or particles alone at noncytotoxic doses. For metabolism, DNA binding and cytotoxicity parameters, the surface area of the particle appeared to play a major role.
NIOSH-Grant; Pulmonary-system-disorders; Pyrenes; Mineral-dusts; Respiratory-system-disorders; Lung-disease; Cell-cultures; Alveolar-cells; Polycyclic-aromatic-hydrocarbons
Environmental Health University of Cincinnati 3223 Eden Ave - M L #56 Cincinnati, OH 45267-0056
50-32-8; 14808-60-7; 7631-86-9; 1309-37-1; 1344-28-1;
Final Grant Report
NTIS Accession No.
Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio
University of Cincinnati, Cincinnati, Ohio