NIOSHTIC-2 Publications Search

Immunological methods for the detection of polycyclic aromatic hydrocarbon-DNA and protein adducts.

Santella RM; Li Y; Zhang YJ; Young TL; Stefanidis M; Lu XQ; Lee BM; Gomes M; Perera FP

Genetic toxicology of complex mixtures. Waters MD, Daniel FB, Lewtas J, Moor MM, Nesnow S, eds. New York: Plenum Press, Environmental Science Research, 1990 Jan; 39::291-301

00227653

The monitoring of exposures to complex mixtures using immunological methods was discussed. Specific topics covered included the measurement of polycyclic aromatic hydrocarbon (PAH)/DNA adducts, the detection of benzo(a)pyrene-diol-epoxide-I (BPDE-1)/protein adducts, determination of serum antibodies against DNA adducts, and determination of multiple adducts. There are several advantages and disadvantages as well. For epidemiological studies where large numbers of samples must be analyzed, the ease and rapidity of immunoassays make them ideal. No radioactivity is used, which is another positive. Generally speaking, even monoclonal antibodies will recognize a number of structurally related adducts. There is the limitation, however, that the authors note that such chemical class specific antisera cannot give absolute quantitative results of the adducts found. Even with this limitation, the authors suggest that immunoassays are useful for the sensitive detection of carcinogen adducts in a number of human populations.

NIOSH-Publication; NIOSH-Grant; Grants-other; DNA-adducts; Protein-chemistry; Bioassays; Carcinogens; Polycyclic-aromatic-hydrocarbons; Biological-monitoring; Risk-analysis; Epidemiology

Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032

EVSRBT

19900101

Conference/Symposia Proceedings

Waters MD; Daniel FB; Lewtas J; Moor MM; Nesnow S;

651727

Grant

1990

9780306436833

Grant-Number-R01-OH-02622

Other Occupational Concerns; Grants-other

Genetic toxicology of complex mixtures

NY; WA

Columbia University New York, New York, New York