Immunological methods for the detection of polycyclic aromatic hydrocarbon-DNA and protein adducts.
Santella RM; Li Y; Zhang YJ; Young TL; Stefanidis M; Lu XQ; Lee BM; Gomes M; Perera FP
Genetic toxicology of complex mixtures. Waters MD, Daniel FB, Lewtas J, Moor MM, Nesnow S, eds. New York: Plenum Press, Environmental Science Research, 1990 Jan; 39::291-301
The monitoring of exposures to complex mixtures using immunological methods was discussed. Specific topics covered included the measurement of polycyclic aromatic hydrocarbon (PAH)/DNA adducts, the detection of benzo(a)pyrene-diol-epoxide-I (BPDE-1)/protein adducts, determination of serum antibodies against DNA adducts, and determination of multiple adducts. There are several advantages and disadvantages as well. For epidemiological studies where large numbers of samples must be analyzed, the ease and rapidity of immunoassays make them ideal. No radioactivity is used, which is another positive. Generally speaking, even monoclonal antibodies will recognize a number of structurally related adducts. There is the limitation, however, that the authors note that such chemical class specific antisera cannot give absolute quantitative results of the adducts found. Even with this limitation, the authors suggest that immunoassays are useful for the sensitive detection of carcinogen adducts in a number of human populations.
NIOSH-Publication; NIOSH-Grant; Grants-other; DNA-adducts; Protein-chemistry; Bioassays; Carcinogens; Polycyclic-aromatic-hydrocarbons; Biological-monitoring; Risk-analysis; Epidemiology
Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032
Waters MD; Daniel FB; Lewtas J; Moor MM; Nesnow S;
Other Occupational Concerns; Grants-other
Genetic toxicology of complex mixtures
Columbia University New York, New York, New York