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Alterations of histone phosphorylation in rat spleen cells after treatment with the aromatic amine, 4,4'-methylene-bis(2-chloroaniline).
DeBord DG; Cheever KL; Booth-Jones AD; Swearengin TF; Savage RE Jr.
J Biochem Toxicol 1995 Feb; 10(1):19-23
A study was conducted on the effects of 4,4'-methylene-bis-(2- chloroaniline) (95534) (MOCA) on histone phosphorylation in rodent spleen cells. Spleen cells isolated from Sprague-Dawley-rats were incubated with up to 50 micromolar (microM) MOCA and the five major histone groups were evaluated using polyacrylamide gel electrophoresis. A significant increase in histone phosphorylation was seen following exposure to 10microM MOCA. MOCA incubation resulted in an initial transient decrease in histone phosphorylation followed by stimulation. The histone H2B group was least affected of all the histones to MOCA induced alterations. The degree of phosphorylation was unaffected by increasing MOCA concentrations with higher concentrations resulting in normal histone phosphorylation values for all histone groups with the exception of H3, which remained elevated following exposure to concentrations greater than 10microM.
NIOSH-Author; Carcinogens; Anilines; Chlorine-compounds; Amines; Protein-biochemistry; Oxidative-phosphorylation; Arylamines
Issue of Publication
Journal of Biochemical Toxicology
Page last reviewed: October 5, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division