Polycyclic aromatic hydrocarbon-DNA and protein adducts in coal tar treated patients and controls and their relationship to glutathione S-transferase genotype.
Santella-RM; Perera-FP; Young-TL; Zhang-J; Chiamprasert-S; Tang-D; Wang-LW; Beachman-A; Lin-H; DeLeo-VA
Mutat Res 1995 Apr; 334(2):117-124
A panel of immunoassays designed to monitor exposure to polycyclic aromatic hydrocarbons (PAH) was evaluated by testing blood samples from coal tar treated patients. Blood samples obtained from coal tar treated psoriasis patients and untreated healthy referents were analyzed using a competitive enzyme linked immunosorbent assay (ELISA) for the detection of DNA and protein adducts with fluorescence endpoint detection, a competitive ELISA with color endpoint detection, and a noncompetitive ELISA for the detection of antibenzo(a)pyrene-diol-epoxide adducts. Elevated levels of PAH-DNA adducts were identified in coal tar treated patients, as determined by ELISA, compared with referents. No associations were seen between days of treatment prior to sample collection or smoking. No differences were seen in level of PAH-albumin adducts between patients and referents. In addition, no relationship could be demonstrated between the genotype for glutathione-S-transferase-M1 and DNA or protein adduct levels. Both patients and referents demonstrated similar titers of antiPAH-DNA adduct antibodies using the noncompetitive ELISA. The authors conclude that an ELISA measuring DNA adducts in white blood cells is the most sensitive method for detecting PAH exposure.
NIOSH-Publication; NIOSH-Grant; Grants-other; Laboratory-techniques; Analytical-methods; DNA-adducts; Polycyclic-aromatic-hydrocarbons; Coal-tar; Pyrenes; Biological-monitoring; Immunological-tests; Blood-samples;
Author Keywords: Coal tar; ELISA; DNA adducts; Protein adducts; Benzo[a]pyrene
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