Urinary biomonitoring for alachlor exposure in commercial pesticide applicators by immunoassay.
Biagini-RE; Tolos-W; Sanderson-WT; Henningsen-GM; MacKenzie-B
Bull Environ Contam Toxicol 1995 Feb; 54(2):245-250
A study was conducted to compare the results of an enzyme linked immunosorbent assay (ELISA) in the detection of the carcinogenic herbicide alachlor (15972608) in urine samples from alachlor exposed pesticide applicators against results obtained via the high performance liquid chromatography (HPLC) analyses of urine hydrolysate for diethylaniline (91667) (DEA). Three urine specimens (prework, postwork and morning after) were collected over a 24 hour period from each of 27 male pesticide applicators and mixers, then analyzed according to the appropriate procedure. The two analytical methods gave statistically significant different results when applied to the specimens that were above the analytical LOD for both methods. The mean result of samples tested by ELISA was 22.5+/-1.79 micromoles/liter alachlor or alachlor equivalents; HPLC mean result was 3.23+/-0.38 micromoles/liter DEA. The two methods showed a significant linear association when subjected to simple orthogonal regression analysis. The authors conclude that, despite unique sensitivity and potential cross reactivity, ELISA methods are a valid addition to instrument based analyses in screening for occupational alachlor exposure. ELISA methods have benefits in comparison to instrumental methods with regards to requiring smaller sample size, time of analysis, cost, sample throughput, and portability.
NIOSH-Author; Agricultural-workers; Occupational-exposure; Herbicides; Urine-chemistry; Chromatographic-analysis; Analytical-methods; Biological-monitoring
Bulletin of Environmental Contamination and Toxicology