Chromosomal "fingerprints" of prior exposure to densely ionizing radiation.
Radiat Res 1994 Oct; 140(1):134-142
The identification of specific chromosomal alterations for use as biomarkers for prior exposure to densely ionizing radiation was described. The use of the ratio of interchromosomal to intrachromosomal exchange type aberrations (F-ratio) as an indication of the type of agent that caused the damage was examined. Spatially inhomogeneous energy depositions resulting from exposure to densely ionizing radiation have been postulated to produce double strand chromosomal breaks that are closer together compared to those induced by sparsely ionizing radiation or chemical agents. Such differences in energy depositions would result in increases in intrachromosomal aberrations relative to interchromosomal aberrations following exposure to densely ionizing radiation and a therefore lower F-ratio. A review of studies supporting the use of the F-ratio as a biomarker of exposure to densely ionizing radiation was presented. Several of these studies reported in-vitro F-ratio values that were consistent with in-vivo reported ratios following exposure to densely ionizing radiation and these values were significantly decreased compared with those seen following exposure to x-rays or gamma rays. An F-ratio of six was suggested as being characteristic of densely ionizing radiation compared with values of about 15 seen using other agents. A two parameter model was described and used to support the conclusions that the experimental F-values were quantitatively consistent with what was known about chromosomal localization and double strand break interaction probabilities.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; DNA-damage; Chromosome-damage; Ionizing-radiation; Radiation-exposure; Radiation-effects; Genotoxic-effects; Biological-monitoring
Radiology Columbia University Health SCi 630 West 168Th St New York, NY 10032
Columbia University New York, New York, New York