Measurement of airborne rat urinary allergen in an epidemiological study.
Gordon-S; Tee-RD; Nieuwenhuijsen-MJ; Lowson-D; Harris-J; Newman Taylor-AJ
Clin Exp Allergy 1994 Nov; 24(11):1070-1077
A study was conducted on the use of radioallergosorbent test (RAST) inhibition for quantitative determination of occupational exposure to rat urinary aeroallergen. Pooled sera from eight people with specific immunoglobulin-E (IgE) to rat urine were incubated with a rat urine standard or test solutions containing rat dust or control air samples. Rat urine discs were incubated with the inhibition mixtures and the discs were then washed, incubated with radiolabeled antihuman IgE, and counted. The interassay and intraassay coefficients of variation at 50% RAST inhibition were 7.0% and 3.0%, respectively, over 1 year which were comparable to those reported for other assays. The assay used was determined to be highly specific for rat urine with mouse urine being over 1,000 times less potent at inhibiting the rat urine RAST system. This assay was used to determine exposure levels of laboratory workers. The grading of exposure to rat urinary aeroallergen in these workers was found to be animal technicians, those who worked with soiled litter, workers exposed to dead animals, and those exposed to rat tissue in the production of histological samples. The authors conclude that the RAST inhibition test is useful for the quantitation of occupational exposure to rats.
Allergic-disorders; Allergic-reactions; Analytical-methods; Laboratory-techniques; Laboratory-workers; Immune-reaction; Radioassays; Occupational-exposure; Laboratory-animals; Epidemiology
Clinical and Experimental Allergy
University of California - Davis