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Activation of complement by silage.
Olenchock-SA; May-JJ; Pratt-DS; Lewis-DM; Mull-JC; Stallones-L
Principles of health and safety in agriculture. Dosman JA, Cockcroft DW, eds., Boca Raton, FL: CRC Press, Inc., 1989 Oct; :116-118
A study was conducted on the interaction between silage and human serum complement in-vitro and in-vivo. Four silage samples were reacted with pooled normal human serum in-vitro. A dose dependent consumption of complement component C3 was seen using extracts from each of the four silage samples tested. There were differences between the samples in the degree of induced complement activity. A correlation was seen between the endotoxin levels in the silage extracts and their complement fixing abilities. All of the extracts were unable to activate complement when the human sera were incubated with EDTA prior to addition of the silage. Pretreatment of the sera with EGTA was not effective in preventing the activation of complement by the samples. Nonsignificant decreases in serum levels of complement components C3 and C4 were seen between blood samples obtained before and after silage dust exposures in nine silo workers along with significant decreases in serum C4 levels in three of the exposed workers who became ill after the exposure.
Organic-dusts; Grain-dusts; Toxic-effects; Dust-exposure; Agricultural-workers; Biological-effects; In-vitro-study; In-vivo-study; Immune-system
Book or book chapter
Principles of health and safety in agriculture
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Content source: National Institute for Occupational Safety and Health Education and Information Division