32P-post-labeling detection of DNA adducts in mononuclear cells of workers occupationally exposed to styrene.
Horvath E; Pongracz K; Rappaport S; Bodell WJ
Carcinogenesis 1994 Jul; 15(7):1309-1315
DNA adduction in mononuclear cells of workers occupationally exposed to styrene (100425) was examined in 47 persons employed at a plastic boat manufacturing facility who were occupationally exposed to styrene. Breathing zone samples were collected at approximately 7 week intervals for 1 year and analyzed for styrene. Blood samples were collected four times during the same year at 3 month intervals. The monocytes were harvested from two of the samplings. The DNA was extracted and analyzed for adducts by the phosphorus-32 (P32) postlabeling/polyethyleneimine thin layer chromatography technique. Breathing zone styrene concentrations measured during the seven surveys varied from 1.4 to 235.4mg/m3, mean 65.6mg/m3. Two adducts having mean relative concentrations of 15.8x10(-8) (adduct 1) and 14.2x10(-8) (adduct 2) were detected. The concentrations of both adducts were significantly linearly related to the breathing zone styrene concentrations, the r-values for adduct 1 and adduct 2 being 0.244 and 0.330, respectively. Cochromatography experiments using standards derived from the reaction of styrene-oxide (96093) with calf thymus DNA enabled identification of adduct 1 as N(2)-(2- hydroxy-1-phenylethyl)-2'-deoxyguanosine-3',5'-bisphosphate. Adduct 2 could not be identified. The authors conclude that occupational exposure to styrene leads to the formation of DNA adducts in mononuclear cells. The extent of adduct formation is linearly related to the concentration of styrene in the ambient air. The extent of adduct formation is not related to an increased level of sister chromatid exchange induction observed previously in lymphocytes of these workers, possibly because of the relatively poor precision of the P32 postlabeling assay.
NIOSH-Publication; NIOSH-Grant; Grants-other; Aromatic-hydrocarbons; Organic-solvents; Occupational-exposure; DNA-adducts; Industrial-hygiene; Breathing-zone; Blood-cells; Boat-manufacturing-industry
Biomedical & Environ Hlth Scis University of California School of Public Health Berkeley, CA 94720
Other Occupational Concerns; Grants-other
University of California Berkeley, Berkeley, California