Influence of particle dose on the cytotoxicity of hamster and rat pulmonary alveolar macrophage in vitro.
Warshawsky-D; Reilman-R; Cheu-J; Radike-M; Rice-C
J Toxicol Environ Health 1994 Aug; 42:407-421
The effects of particle size distribution and surface area on the cytotoxicity of alveolar macrophages (AMs) was studied. Crystalline- silica (14808607), gelled-silica (7699414), precipitated-silica, fumed-silica (7631869) ferric-oxide (1309371), and aluminum-oxide (1344281) were evaluated and sized by scanning electron microscopy, automatic surface area analysis, and X-ray diffraction. AMs were isolated from Sprague-Dawley-rats and golden-Syrian-hamsters by tracheal lavage. Cultures of AMs were exposed to different concentrations of particles for 24 and 48 hours. The cells were then washed, and viability was assessed by erythrosin-B dye exclusion. The results indicated that the median diameter for aluminum-oxide, ferric-oxide, and amorphous silicas was equal to or less than 0.38 micrometer and 0.83 micrometer for crystalline silica. Surface area for amorphous silicas and aluminum-oxide ranged from 253 to 125 square meters per gram and 4.3 to 10.8 square meters per gram for crystalline-silica and ferric-oxide. Ferric- oxide and aluminum-oxide were not cytotoxic, gelled-silica was cytotoxic, and precipitated-silica and fumed-silica were intermediately cytotoxic at equivalent doses. The authors conclude that the dose, surface area, and surface characteristics of particles are important determinants in the cytotoxicity of hamster and rat AMs.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Cancer; Lung-cells; Phagocytes; Cytotoxic-effects; Cell-damage; Silicates; Aluminum-compounds; Iron-compounds; In-vitro-studies
Environmental Health University of Cincinnati 3223 Eden Avenue Cincinnati, OH 45267-0056
14808-60-7; 7699-41-4; 7631-86-9; 1309-37-1; 1344-28-1
Journal of Toxicology and Environmental Health
University of Cincinnati, Cincinnati, Ohio