Effects of taurine and ketamine on bovine retinal membrane lipid peroxidation.
Zhou-M; Ma-T; Tseng-MT
Neuroscience 1991 Jan; 45(2):461-465
The effects of the ischemic protectants taurine (107357) (TA) and ketamine (6740881) (KE) on bovine retinal membrane lipid peroxidation were investigated. Bovine eyes and retinas were subjected to ischemia. Retinal cells or rod outer segments were isolated. The tissues were exposed to the lipid peroxidation inducers cadmium-chloride (10108642) (CC) and L-ascorbic-acid (50817) (AA) in the presence of TA (0 to 5 millimolar (mM)), KE (0 to 10mM), kynurenic acid (492273) (1mM), dextromethorphan (125713) (1mM), or trifluoperazine (117895) (TFP). Lipid peroxidation was measured by the thiobarbiturate assay. Results showed that 30 minutes (min) exposure to CC or AA increased the basal thiobarbituric-acid reactive substance value of lipid peroxidation to 0.637 and 0.457OD/mg protein, respectively, from a basal value of 0.273OD/mg protein. TFP caused a dose dependent reduction in lipid peroxidation. No significant difference was seen with low (0.1 or 1.0mM) KE, although 9mM or 10mM KE significantly reduced it. TA failed to alter lipid peroxidation of P2 membranes. Similar effects were found with isolated retinal cells. With intact eyes, KE reduced lipid peroxidation to 55% control values, but TFP was less effective, and TA failed to prevent lipid peroxidation of the retinal membrane. KE prevented glutathione depletion during in- vitro ischemia, but TA was without effect. The authors conclude that different mechanisms seem to underlie the ischemic protective properties of TA and KE as ischemic protectants, and that lipid peroxidation may not be critical to the antihypoxic property of TA.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Eye-damage; Eye-protection; Free-radicals; Hypoxia; In-vitro-study; Membrane-dysfunction; Metabolic-study; Lipid-peroxidation
Anatomy University of Louisville Anatomy and Neurobiology Louisville, KY 40292
107-35-7; 6740-88-1; 10108-64-2; 50-81-7; 492-27-3; 125-71-3; 117-89-5
Neurotoxic Disorders; Neurotoxic-effects
University of Louisville, Louisville, Kentucky