In vitro pharmacologic studies on poultry dust extract.
Schachter-EN; Zuskin-E; Rienzi-N; Godbold-J
1994 Proceedings, Beltwide Cotton Conferences, San Diego, California, January 5-8, 1994, National Cotton Council of America, Memphis, Tennessee 1994 Jan; 1:365-366
In-vitro pharmacological studies were conducted on poultry dust extract. A water soluble extract of poultry dust (PDE) was prepared as a 1:10 weight/volume solution. Tracheal rings (TR) were obtained from male Hartley-guinea-pigs sacrificed by carbon-dioxide narcosis. TR isometric concentrations were measured using a polygraph recorder. After the TR were equilibrated and contracted with carbachol, tissues were thoroughly washed until total relaxation was achieved. Tissues were then treated with Krebs solution (control), indomethacin, pyrilamine, and LY171883 for Series-I, and with Krebs solution, captopril and thiorphan in Series-II. Tissues were incubated for 15 minutes, and a dose response challenge was performed with PDE. Results showed that there was a typical dose response relationship between PDE and the contractile response. The maximum mean contraction effect of PDE was 10% maximal carbachol contraction, with 1,000 microliters (microl) of PDE after incubation with Krebs. All the drugs lowered tracheal contraction. Differences in mean response between control and drug treatment were statistically significant for thiorphan (30microl), LY171883 (100microl) and for indomethacin, pyrilamine, and captopril (300microl). The authors conclude that PDE causes a dose dependent contraction of guinea-pig trachea, and that enzyme inhibitors reduce the PDE contractile effect.
NIOSH-Grant; Pulmonary-system-disorders; In-vitro-studies; Respirable-dust; Dose-response; Laboratory-animals
Medicine Mount Sinai Medical Center One Gustave L Levy Place New York, N Y 10029
1994 Proceedings, Beltwide Cotton Conferences, San Diego, California, January 5-8, 1994, National Cotton Council of America, Memphis, Tennessee
Mount Sinai School of Medicine, New York, New York