Structural equation modeling and nested ANOVA: effects of lead exposure on maternal and fetal growth in rats.
Hamilton-JD; O'Flaherty-EJ; Ross-R; Shukla-R; Gartside-PS
Environ Res 1994 Jan; 64(1):53-64
The effects of lead (7439921) exposure on maternal and fetal growth were examined using structural equation modeling and nested analysis of variance (ANOVA) in rats. Lead-acetate (301042) was administered in drinking water to female Sprague-Dawley-rats ad-libitum beginning at day 25 of age. Doses ranged from 250 to 1,000 parts per million lead. Lead exposure had a direct negative effect on body weight and growth (tail length) during the first week of exposure. During the next 2 weeks of exposure, a high correlation between growth indices and food intake over time resulted in an apparent reduction in early postnatal growth. These effects were reversible, since reduced growth was not evident beyond the third week of exposure. Mating began after 8 weeks of exposure and exposure continued during the gestation period. Lead exposure was associated with a decrease in fetal body weight when effects of litter size, intrauterine position, and sex were controlled in a nested ANOVA. The authors conclude that lead exposure did not appear to affect fetal skeletal development, possibly because lead did not alter levels of maternal serum calcium and phosphorus.
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; Analytical-methods; Reproductive-hazards; Lead-compounds; Laboratory-animals; Long-term-exposure; Growth-rate; Transplacental-exposure; Developmental-disorders
Environmental Health University of Cincinnati 3223 Eden Avenue Cincinnati, OH 45267
University of Cincinnati, Cincinnati, Ohio