Plasma Proteins: Markers of Chemical Exposure.
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, University of Texas, Galveston, Texas 1994:7 pages
The use of plasma proteins as markers of chemical exposure was studied. The specific areas of investigation were the covalent binding of acrolein (107028) to albumin, the rapid quantitation of acrolein and crotonaldehyde (4170303) modified albumin, the susceptibility to degradation of covalently modified albumin, the development of an enzyme linked immunosorbent assay (ELISA) for styrene (100425), and the preparation and detection of styrene-oxide (96093) specific antibodies. The covalent binding of acrolein to human serum albumin showed four new ninhydrin positive peaks. Study of the effects of acrolein on the ultraviolet absorption and the biological function of albumin to bind fatty acid or bromocresol- green, showed that absorption was increased by approximately 80% at 280 nanometers. The authors suggest that more tyrosine and/or tryptophan residues were exposed as a result of the unfolding of the protein through covalent modification of the lysyl and histidyl residues. The preparation of a styrene-oxide albumin conjugate, the generation of polyclonal antibodies in rabbits, and an ELISA procedure for the detection of antibodies were also discussed.
NIOSH-Grant; Grants-other; Cytotoxic-effects; Protein-chemistry; Laboratory-animals; In-vivo-studies; In-vitro-studies; Blood-plasma; Biological-monitoring;
Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
107-02-8; 4170-30-3; 100-42-5; 96-09-3;
Final Grant Report;
NTIS Accession No.
Other Occupational Concerns; Grants-other;
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, University of Texas, Galveston, Texas
University of Texas Medical Branch, Galveston, Texas