Biological monitoring for occupational exposures to o-toluidine and aniline.
Teass-AW; DeBord-DG; Brown-KK; Cheever-KL; Stettler-LE; Savage-RE; Weigel-WW; Dankovic-D; Ward-E
Int Arch Occup Environ Health 1993 Jan; 65(1):S115-S118
Methods for the biological monitoring of occupational exposure to o- toluidine (95534) and aniline (62533) were developed and tested. Levels of o-toluidine and aniline in urine were determined by high performance liquid chromatography followed by electrochemical detection. Limits of detection were 0.6 micrograms per liter for o- toluidine and 1.4 micrograms per liter for aniline. Recovery was approximately 86 to 93% over a range of 4 to 100 micrograms per liter. Application of this method to preshift and postshift samples collected from potentially exposed and unexposed workers revealed elevated concentrations of aromatic amines in the urine of exposed workers. An alternative approach involved the sampling of blood for analysis of protein adducts serving as biomarkers of internal dose. Results of in-vivo (rodents) and in-vitro studies indicated that hemoglobin adducts were more useful than albumin adducts as measures of o-toluidine exposure when analyzing for base hydrolyzable protein adducts. Gas chromatography with electron capture was more sensitive in the determination of o-toluidine hemoglobin adducts than high performance liquid chromatography with fluorescence, but the presence of interfering peaks was a limiting factor.
NIOSH-Author; Biological-monitoring; Analytical-methods; Anilines; Occupational-exposure; Urinalysis; Chromatographic-analysis; Carcinogens; Workplace-studies; Toluidines
International Archives of Occupational and Environmental Health