Exposure of rats to hyperoxia: alteration of lavagate parameters and macrophage function.
Dedhia-HV; Ma-JY; Vallyathan-V; Dalal-NS; Banks-D; Flink-EB; Billie-M; Barger-MW; Castranova-V
J Toxicol Environ Health 1993 Sep; 40(1):1-13
A study was conducted on the pulmonary response of Sprague-Dawley- rats to exposure to hyperoxia. Animals were exposed to an atmosphere of 100% oxygen (7782447) for 64 hours and bronchoalveolar lavage fluid was obtained immediately after termination of the exposure. Decreases in the percentage of alveolar macrophages and increases in the percentages of red blood cells, neutrophils, and lymphocytes were seen following the exposure. In addition a significant increase was noted in the protein content of the lavage fluid. Hyperoxia induced a significant decrease in the ability of alveolar macrophages to secrete hydrogen-peroxide and produce oxygen radicals in response to stimulation by zymosan. A significant decrease was seen as well in the ability of the macrophages to exclude trypan-blue dye. The oxygen exposed macrophages had a greater chemiluminescence response to zymosan compared with controls, and lipid peroxidation in these cells was significantly increased as well.
NIOSH-Author; Toxic-effects; Oxygen-toxicity; Lung-function; Alveolar-cells; Oxidative-processes; Laboratory-animals
Journal of Toxicology and Environmental Health