The effects of inhalation of grain dust extract and endotoxin on upper and lower airways.
Clapp-WD; Thorne-PS; Frees-KL; Zhang-X; Lux-CR; Schwartz-DA
Chest 1993 Sep; 104(3):825-830
The effects of grain dust extracts and a bacterial endotoxin on airway function and inflammation were studied in seven volunteers. Three men and four women, mean age 26.9 years, inhaled nebulized corn and soybean dust extracts, lyophilized Escherichia-coli (E- coli) endotoxin diluted in Hanks balanced salt solution (HBSS), or HBSS in a randomized double blind crossover design. The interval between each inhalation challenge was at least 10 days. Spirometric testing, nasal lavage, and peripheral blood tests were performed before and after each challenge. One second forced expiratory volume (FEV1) values were significantly decreased at most times 0.5 to 7 hours after each challenge. The largest decrease occurred after challenge with the endotoxin solution, followed by the corn and soybean dust extracts and HBSS. Forced vital capacity (FVC) was significantly decreased 1 to 5 hours after endotoxin challenge, 2 to 24 hours after corn dust, and 24 hours after soybean challenge. The FEV1/FVC ratio was significantly decreased 0.5 hour after challenge with the grain dust extracts and endotoxin. Peripheral blood leukocyte and neutrophil counts were significantly increased after each dust and endotoxin challenge. Peripheral blood lymphocytes were significantly decreased only after corn dust challenge. The only significant change in nasal lavagate cellularity was an increase in lymphocyte counts following challenge with the corn dust extract. The authors conclude that inhalation of grain dust extracts causes a mild airflow limitation that is similar in severity and duration to that occurring after endotoxin inhalation.
NIOSH-Grant; NIOSH-Cooperative-Agreement; Pulmonary-system-disorders; Grain-dusts; Endotoxins; In-vivo-studies; Men; Women; Inhalation-studies; Pulmonary-function-tests; Blood-cells; Nasal-cavity; Physiological-response
Internal Medicine University of Iowa Pulmonary Disease Division Iowa City, IA 52242
Grant; Cooperative Agreement
Pulmonary System Disorders
University of Iowa, Iowa City, Iowa