Benzamide on chondrocytic differentiation in chick limb bud cell culture.
J Embryol Exp Morphol 1985 Feb; 85(1):163-175
In order to understand the mechanisms by which inhibitors of (ADP- ribose) transferase augment chondrocytic differentiation, the changes in cell growth, sulfated proteoglycan synthesis, DNA synthesis, RNA synthesis, (ADP-ribose) transferase activity, and intracellular NAD and ATP in cultured chick limb bud mesenchymal cells were studied. Chondrocytic differentiation of chick limb bud mesenchymal cells in micromass cultures was augmented by benzamide (55210). Sulfate incorporation into the trichloroacetic-acid insoluble constituents of cell masses occurred about 24 hours earlier than in untreated cultures. Similarly, the formation of cartilage nodules occurred more rapidly in cultures of stage 23 and 24 chick limb bud cells. A significant increase was also noted in cell proliferation caused by benzamide exposure. No affect was noted on DNA and RNA syntheses except for the 24 to 30 hour period after the start of the culture when RNA synthesis was stimulated. In the untreated cultures, from hour 48 onward, (ADP-ribose) transferase activity decreased each day. Benzamide treatment caused a lessening in the (ADP-ribose) transferase activity to occur 24 hours earlier. An increase was noted in intracellular NAD levels in the untreated cultures each day, and benzamide treatment significantly increases these levels. No difference was noted in the ATP levels during the study period in the untreated or the benzamide exposed cultures.
NIOSH-Publication; NIOSH-Grant; Grants-other; Enzyme-activity; Cell-cultures; Cytotoxic-effects; Amides; In-vitro-studies
Author Keywords: Chondrocytes; differentiation; proliferation; (ADP-ribose) transferase; nicotinamide adenine dinucleotide
Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kansas 66103
Other Occupational Concerns; Grants-other
Journal of Embryology and Experimental Morphology
University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas