Cellular f-actin levels as a marker for cellular transformation: correlation with bladder cancer risk.
Rao-JY; Hemstreet-GP III; Hurst-RE; Bonner-RB; Min-KW; Jones-PL
Cancer Res 1991 Jun; 51(11):2762-2767
A risk stratification scheme was used to investigate the possibility of using quantitative F-actin measurements in urinary cells to provide diagnostic or prognostic information by identifying those individuals with cells tending towards a lower degree of differentiation. Bladder wash samples were taken from 163 symptomatic patients undergoing evaluation for bladder cancer and 41 asymptomatic comparisons with no hematuria or other symptoms consistent with bladder cancer. Flow cytometry using a fluorescent phallodin probe was used to evaluate F-actin levels. The risk of bladder cancer was stratified according to biopsy, either DNA ploidy by flow cytometry or quantitative fluorescence image analysis cytology, previous bladder cancer history, and hematuria. A strong correlation was noted between the presence of cells with abnormally low F-actin content in cells obtained by bladder wash from 38 patients and biopsy proved bladder transitional cell carcinoma. The presence of cells with low F-actin content was also highly correlated to risk of bladder cancer assessed by either stratification schema. Because of the 37% false positive rate of ploidy analysis by flow cytometry among the comparison patients, the correlation was more consistent with the stratification by quantitative fluorescence image analysis cytology. The F-actin content of the M344-positive cells was lower than that of the M344- negative cells. These findings suggested that F-actin could be an early and sensitive marker for bladder cancer detection and risk prognostication.
NIOSH-Publication; NIOSH-Grant; Cancer; Cancer-rates; Risk-analysis; Humans; Bladder-cancer; Epidemiology; Cell-damage; Cell-transformation
Urology University of Oklahoma Dept of Urology, PO Box 26901 Oklahoma City, OK 73190
University of Oklahoma Hlth Sciences Ctr, Oklahoma City, Oklahoma