Modulation of the population density of identifiable epidermal Langerhans cells associated with enhancement or suppression of cutaneous immune reactivity.
J Immunol 1986 Feb; 136(3):867-876
A study was conducted to determine whether increased numbers of identifiable Langerhans cells were associated with an enhancement of cutaneous immune reactivity and whether depression of identifiable Langerhans cells after application of high doses of arachidonic-acid (506321) (AA) was associated with decreased cutaneous immune reactivity. Male DBA/2-mice were treated on days one through seven by topical application of 100 microliters of 2% or 0.5% AA to either the back or the dorsal surface of the ear. Skin biopsies were obtained on day eight, and the population density of Langerhans cells was determined. AA demonstrated a biphasic effect on epidermal Langerhans cells. In low doses, it increased their number; in high doses, it decreased their number. A correlation was noted between the increased population density of identifiable epidermal Langerhans cells induced with AA and an increase in afferent and efferent immune reactivity. Reduction of Langerhans cells with larger amounts of AA suppressed the afferent and efferent limb of the immune response. 2,4-dinitro-1-fluorobenzene applied to skin with decreased Langerhans cell density from AA caused a state that mimicked immune tolerance. This method was able to either increase or decrease the population density of Langerhans cells and modulate up or down the afferent or efferent limbs of the cutaneous immune response. The authors suggest that the Langerhans cell may be involved in the efferent limb of the immune efferent response.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Immune-reaction; Immune-system-disorders; Cell-damage; Skin-exposure; Metabolic-study; Allergic-reactions; Allergic-disorders; Laboratory-animals
Dermatology University of Cincinnati 231 Bethesda Avenue Cincinnati, OH 45267
The Journal of Immunology
University of Cincinnati, Cincinnati, Ohio