Rapid induction of Thy-1 antigenic markers on keratinocytes and epidermal immune cells in the skin of mice following topical treatment with common preservatives used in topical medications and in foods.
Rheins LA; Young EM; Nordlund ML; Berning RB; Nordlund JJ
J Invest Dermatol 1987 Nov; 89(5):489-494
A study was conducted to determine whether topical application of certain compounds could change the density, function, and expression of Ia+ Langerhans cells (LC) or Thy-1+ dendritic epidermal cells (DEC) or cause increased expression of Ia and Thy-1 on keratinocytes. Butylated-hydroxytoluene (128370) (BHT) or butylated- hydroxyanisole (25013165) (BHA) was applied to the shaved back or dorsal surface of the ear of male DBA/2d-mice, C57BL/6b-mice, or Balb/C4-mice on days one through five. Skin biopsies were obtained on day six, for the population density of LC and Thy-1 DEC. BHT and BHA were shown to significantly increase the population density of Thy-1+ DEC within 5 days. No effect was noted on Ia+ cells. The authors suggest that the Thy-1 DEC cells may be more mobile and/or their surface markers may be readily expressed and not a slowly mobile population. These parasubstituted phenolic compounds appear to act like pertussis-toxin and induce Thy-1 and Ia+ expression in keratinocytes. No accompanying functional alterations were noted in the immune response to contact allergens.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Allergic-reactions; Skin-exposure; Food-additives; Laboratory-animals
Dermatology University of Cincinnati 231 Bethesda Avenue Cincinnati, OH 45267
Journal of Investigative Dermatology
University of Cincinnati, Cincinnati, Ohio