Influence of the dose levels of cocarcinogen ferric oxide on the metabolism of benzo(a)pyrene by pulmonary alveolar macrophages in suspension culture.
J Toxicol Environ Health 1993 Apr; 38(4):399-417
The effects of the phagocytosis of ferric-oxide (1309371) (FeO) by alveolar macrophages on the metabolism of benzo(a)pyrene (50328) (BaP) and on the production of reactive oxygen species were studied in-vitro. Alveolar macrophages were harvested by tracheal lavage from Syrian-Golden-hamsters, cultured with dimethylsulfoxide and cytochrome-c, and exposed to radiolabeled BaP that had previously been adsorbed onto FeO. A decrease in cell viability was seen that was dependent upon the dose of BaP coated particles present in the culture media. A significant increase in the amount of reduced cytochrome-c was seen up to 6 hours after the addition of BaP coated FeO or FeO alone compared with the amount reduced in untreated cells or cells incubated with BaP alone. The amount of reduced cytochrome- c plateaued in all preparations after 6 hours. The highest concentration of phagocytosed BaP was seen in cultures exposed to BaP coated FeO at 3 hours. The phagocytosed BaP was metabolized by the macrophages and the total concentration of metabolites produced appeared to be dependent on the dose of FeO. Significantly more metabolites were found in cells exposed to BaP coated FeO compared with those exposed to BaP alone.
NIOSH-Publication; NIOSH-Author; NIOSH-Grant; Pulmonary-system-disorders; Carcinogens; Metabolic-study; Lung-cells; Pyrenes; Oxidative-processes; In-vitro-study; Dose-response; Mammalian-cells
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Journal of Toxicology and Environmental Health
University of Cincinnati, Cincinnati, Ohio