The effects of gamma radiation on chondrogenic development in vitro.
Radiat Res 1988 Aug; 116(2):356-363
The effect of gamma irradiation on chick embryo limb bud chondrogenesis was studied in-vitro. Limb bud cells obtained from White-Leghorn-chicken embryos were cultured in the presence or absence of benzamide (55210) and irradiated with 0 to 8.0 gray cesium-137 gamma rays 2, 24, 48, or 72 hours after plating. The effects on chondrogenesis were assessed by measuring the extent of cellular proliferation and cartilage nodule formation and the amounts of Alcian-blue dye bound to the cultures. Staining with Alcian-blue was determined spectrophotometrically and by computer assisted microscopy. Benzamide significantly stimulated cell proliferation and cartilage nodule formation. Gamma irradiation caused significant dose related decreases in cartilage nodule formation 2 and 24 hours after plating. Cell proliferation was significantly inhibited 2 to 48 hours after plating. These effects occurred in both benzamide treated and untreated cells. Alcian-blue staining was significantly inhibited by gamma irradiation 2 to 48 hours after plating. The extent of staining determined spectrophotometrically and by computer assisted microscopy was similar. The authors conclude that gamma irradiation inhibits chondrogenesis in chick limb bud cell cultures. The fact that cartilage nodule formation is inhibited only during the first 24 hours after plating suggests that chondrocyte commitment occurs during that period of development.
NIOSH-Publication; NIOSH-Grant; Grants-other; In-vitro-studies; Embryology; Gamma-radiation; Amides; Histochemical-analysis; Microscopic-analysis; Radiation-effects
Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kans 66103
Other Occupational Concerns; Grants-other
University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas