Field molecular epidemiology. Feasibility of monitoring for the malignant bladder cell phenotype in a benzidine-exposed occupational cohort.
Bi WF; Rao JY; Hemstreet GP; Fang P; Asal NR; Zang M; Min KW; Ma Z; Lee E; Li G; Hurst RE; Bonner RB; Wang Y; Fradet Y; Yin S
J Occup Med 1993 Jan; 35(1):20-27
Quantitative biochemical and cytological markers were used in a pilot study to quantitate the effects of benzidine (92875) exposure in a well characterized cohort of workers. Three groups were randomly selected and included a group of 23 bladder cancer cases previously exposed to benzidine, a group of 20 subjects with previous exposure history but not previously diagnosed with bladder cancer, and an unexposed comparison group. Biochemical markers included quantitative fluorescence image analysis cytology (QFIA), conventional urinary cytology, the p300 tumor associated antigen detected with the M344 monoclonal antibody, and p185. Samples were collected in China, and analyzed in the US up to 1 week later. Occupational, medical, and smoking histories were obtained on these workers to identify exogenous risk factors. The results of the pilot study indicated that even in remote locations it was possible to obtain useful data and urine samples for analysis. Abnormal findings were found among the 20 subjects exposed but not diagnosed with cancer by both QFIA cytology and p300 expression and these were later diagnosed as having bladder cancer. It was possible also to detect premalignant changes related to exposure to benzidine. The authors conclude that the feasibility of performing molecular marker field studies to define risks in exposed worker cohorts was demonstrated conclusively by these findings.
JOCMA7; NIOSH-Publication; NIOSH-Grant; Carcinogens; Carcinogenesis; Urinalysis; Aromatic-hydrocarbons; Urine-chemistry; Urogenital-system-disorders; Biological-monitoring; Humans; Occupational-exposure; Bladder-cancer; Benzidines
Urology University of Oklahoma Dept of Urology, PO Box 26901 Oklahoma City, OK 73190
Journal of Occupational Medicine
University of Oklahoma Hlth Sciences Ctr, Oklahoma City, Oklahoma