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Pulmonary response of the guinea pig animal model to n-formyl- methionyl-leucyl-phenylalanine (FMLP) liquid aerosol.

Frazer DG; Robinson VA; Weber KC; Jones W; Siegel PD; Barger MW; Masters B; Chandler CA; Vincent D; Castranova V
Cotton dust: proceedings of the Sixteenth Cotton Dust Research Conference, beltwide cotton conferences, January 9-10, 1992, Nashville, Tennessee. Domelsmith LN, Jacobs RR, Wakelyn PJ, eds. Memphis, TN: The National Cotton Council of America, 1992 Jan; :266-270
Pulmonary responses to n-formyl-methionyl-leucyl-phenylalanine (59880976) (FMLP) were studied in guinea-pigs. English-short-hair- guinea-pigs were exposed to an aerosol consisting of 1mg/m3 FMLP in phosphate buffer for 4 hours. Controls breathed the phosphate buffer or filtered air. Selected animals were killed immediately after exposure and the lungs were removed. The volume of trapped gas in the lungs was determined as a marker of airway obstruction. Breathing rates of the remaining animals were measured in air or air containing 10% carbon-dioxide before and at 3 hour intervals after exposure for up to 18 hours. The animals were then killed and the lungs were removed and lavaged. Lavage fluid cellularity was determined. The alveolar macrophages were isolated and their ability to release superoxide with or without stimulation by zymosan was evaluated. FMLP or the phosphate buffer did not alter the volume of trapped gas. FMLP caused a significant increase in breathing rate in the carbon-dioxide containing mixture. The maximum increase was observed immediately after exposure. The breathing rate decreased at later times but was still elevated above the control value at 18 hours postexposure. FMLP caused a significant increase in lavage fluid total cell, granulocyte, lymphocyte, and red blood cell counts compared to the phosphate buffer and air breathing controls. FMLP increased the extent of superoxide release by zymosan stimulated alveolar macrophages relative to the controls. The authors conclude that guinea-pigs respond to FMLP liquid aerosols, but the response differs from that induced by exposure to cotton dust.
In-vivo-studies; Laboratory-animals; Peptides; Inhalation-studies; Pulmonary-function; Alveolar-cells; Acute-exposure; Laboratory-techniques; Cotton-dust
Publication Date
Document Type
Conference/Symposia Proceedings
Domelsmith LN; Jacobs RR; Wakelyn PJ;
Fiscal Year
Source Name
Cotton dust: proceedings of the Sixteenth Cotton Dust Research Conference, beltwide cotton conferences, January 9-10, 1992, Nashville, Tennessee
Page last reviewed: February 25, 2022
Content source: National Institute for Occupational Safety and Health Education and Information Division