Effect of animal weight on the response of the guinea pig model to inhalation of cotton dust.
Robinson-VA; Castranova-V; Barger-MW; Frazer-DG
Cotton dust: proceedings of the Sixteenth Cotton Dust Research Conference, beltwide cotton conferences, January 9-10, 1992, Nashville, Tennessee. Domelsmith LN, Jacobs RR, Wakelyn PJ, eds. Memphis, TN: The National Cotton Council of America, 1992 Jan; :259-262
The effects of body weight on the response to cotton dust were studied in guinea-pigs. English-short-hair-guinea-pigs having mean body weights of 261.1, 352.9, and 504.9 grams (g) were exposed to 11.6mg/m3 aerosolized cotton dust or clean air for 6 hours. Selected animals were killed immediately after exposure to determine lung residual gas volume, a measure of airway closure. Breathing rates of the remaining animals were measured in air or 10:90 carbon- dioxide/air before and at various times up to 18 hours postexposure. The animals were then killed and the lungs were removed and lavaged. Lavage fluid cellularity was determined. The alveolar macrophages were isolated and the extent of superoxide release in response to stimulation by unopsonized zymosan was determined. Cotton dust exposure induced increases in breathing rate, increases in the number of lavageable total cells, granulocytes, and lymphocytes, release of macrophage superoxide, and increased airway closure. The increases in breathing rate in the 504.5g guinea-pigs were significantly lower than in animals weighing 266.1 and 352.9g. Influx of granulocytes into the lavage fluid increased with increasing body weight. The other parameters were not significantly affected by body weight. The authors conclude that when using guinea-pigs in acute studies of byssinosis, the effects of animal size must be considered.
Cotton-dust; In-vivo-studies; Laboratory-animals; Inhalation-studies; Body-weight; Pulmonary-function; Alveolar-cells; Physiological-response
Domelsmith-LN; Jacobs-RR; Wakelyn-PJ;
Cotton dust: proceedings of the Sixteenth Cotton Dust Research Conference, beltwide cotton conferences, January 9-10, 1992, Nashville, Tennessee