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The immunopathogenesis of occupational disease due to reactive chemicals.

Bernstein-DI

Division of Immunology, University of Cincinnati Medical Center, Cincinnati, Ohio 1992 Jul; :1-25

00211769

The role of cellular immune mechanisms in the onset and development of occupational asthma resulting from exposure to reactive chemicals was investigated. An assay was developed to measure histamine releasing factor (HRF) produced in the peripheral blood mononuclear cells (PBMC) of workers exposed to diisocyanates after in-vitro coincubation with diisocyanate protein antigens. The PBMCs from workers with confirmed occupational asthma demonstrated higher spontaneous HRF production than did workers with suspected diisocyanate caused occupational asthma. Mean HRF activity produced by PBMC following stimulation with a human serum albumin/diisocyanate conjugate was greatly increased in the group with confirmed asthma from diisocyanate exposure. No serum specific immunoglobulin-E was found in this group of workers. The investigators conclude that the mechanisms which are responsible for the production of HRF are independent of classical mechanisms of immediate hypersensitivity.

NIOSH-Grant; Pulmonary-system-disorders; Blood-cells; Bronchial-asthma; Occupational-exposure; Humans; Enzyme-activity; Respiratory-system-disorders; Allergic-reactions

Internal Medicine University of Cincinnati Med C 231 Bethesda Ave. Cincinnati, Ohio 45267-0563

19920707

Final Grant Report

97200

Grant

1992

PB93-159382

A03

Grant-Number-K01-OH-00073

OEP

Respiratory-system-disorders

Division of Immunology, University of Cincinnati Medical Center, Cincinnati, Ohio

OH

University of Cincinnati, Cincinnati, Ohio