Covalent binding of ethylene dibromide and its metabolites to albumin.
Toxicol Lett 1992 Sep; 62(2-3):221-230
Male Sprague-Dawley-rats were used to evaluate the potential of ethylene-dibromide (106934) (EDB) modified albumin as a marker of EDB exposure. Rats were treated with 25mg/kg EDB for 12 consecutive days and sacrificed 24 hours after the last dose. Plasma derived from blood samples was examined by size exclusion high performance liquid chromatography (SEHPLC). Results revealed elution of a radioactive peak at an elution volume corresponding to 65,000 dalton molecular mass. In-vitro analysis involved incubation of human plasma or purified albumin with EDB in the presence of phenobarbital treated rat liver microsomes and an NADPH generating system for 2 hours. Approximately 0.28 nanomole equivalent EDB/milligram protein was associated with albumin. This was twice that measured for the in-vivo binding. Affinity chromatography of albumin fraction isolated by SEHPLC further supported the binding of the carbon-14 label to albumin under in-vivo and in-vitro conditions. Several amino acid adducts of EDB and/or its metabolites were formed. The authors suggest that structural elucidation will help to develop a relatively noninvasive method of measuring EDB exposure.
NIOSH-Publication; NIOSH-Grant; Grants-other; Fumigants; Agricultural-chemicals; Bromides; Metabolic-study; Pesticides; Laboratory-animals; In-vivo-study; In-vitro-study;
Author Keywords: Ethylene dibromide; Covalent binding; Albumin
Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
Other Occupational Concerns; Grants-other
University of Texas Medical Branch, Galveston, Texas