Immuno-based Personal Exposure Monitors.
Drinkwine-A; Spurlin-S; Emon-J; Lopez-Avila-V
NIOSH 1991 Feb:449-459
An attempt was made to develop personal exposure monitors (PEMs) that use either a polyclonal or a monoclonal antibody immobilized onto a silica support for the collection and detection of a specific target analyte or compound class. A system was being developed which would result in a compact, diffusion based personal exposure monitor for specific target analysis. Semipermeable membrane tubes with a high surface to volume ratio were used to overcome the interface problem of the aqueous based antibody system. For pentachlorophenol (87865) (PCP), an immunobased collection and analysis system using a monoclonal antibody was investigated. Monitoring limits were constrained by the detection capability of the antibody based system. The limit of detection for the PEM device should be from 1 to 5 nanograms of PCP. This would convert to a minimum exposure time of around 20 minutes for the analyte to reach a detectable quantity within the PEM device based on a vapor concentration of 5 parts per billion (ppb). Early results indicated that it will be possible to apply antibodies to direct air monitoring systems through the use of microdialysis tubing as a semipermeable barrier which allows vapor diffusion without significant moisture loss.
Air-quality-monitoring; Monitoring-systems; Chlorinated-phenols; Air-monitoring; Environmental-contamination; Work-environment; Occupational-exposure;
Field Screening Methods for Hazardous Wastes and Toxic Chemicals. Second International Symposium, February 12-14, 1991. Sponsored by U.S. EPA; U.S. DOE; U.S. Army Toxic and Hazardous Materials Agency; U.S. Army Chemical Research Development and Engineering Center; U.S.A.F.; Florida State Univ.; National Environmental Technology Applications Corp.; and NIOSH