Evaluation of the V79 cell metabolic co-operation assay as a screen in vitro for developmental toxicants.
Toraason-M; Bohrman-JS; Krieg-E; Combes-RD; Willington-SE; Zajac-W; Langenbach-R
Toxicol In Vitro 1992 Mar; 6(2):165-174
The utility of the V79 cell metabolic cooperation assay as an in- vitro screen for developmental toxicants was evaluated. Thirty- eight coded chemicals were tested for their effect on intercellular communication. A separate cytotoxicity assay was used to determine the concentration of each chemical that inhibited clonal expansion of V79 cells. Only seven of the 29 designated teratogens proved positive for inhibition of intercellular communication. Four teratogens and one nonteratogen inhibited intercellular communication at only a single concentration or at cytotoxic concentrations and were considered equivocal. The sensitivity of the assay for teratogens was therefore in the range of 24 to 38%. The overall accuracy for correctly identifying teratogens and nonteratogens was in the range of 42 to 50%. Specificity ranged from 89 to 100%. The authors conclude that chemicals which inhibit intercellular communication under the given conditions have a high probability of being teratogens. Reasons for reduced accuracy of the assay were considered.
NIOSH-Publication; NIOSH-Author; NIOSH-Contract; Contract-200-84-2783; Contract-IA-85-14; Bioassays; Analytical-methods; Screening-methods; In-vitro-study; Cell-function; Toxic-materials; Laboratory-testing
M. Torrason, Cellular Toxicology Section C23, DBBS, NIOSH, CDC, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
Interagency Agreement; Contract
Toxicology in Vitro