Cytogenetic effects of vincristine sulfate and ethylene dibromide in human peripheral lymphocytes: micronucleus analysis.
Environ Mol Mutagen 1992; 20(2):117-126
Micronuclei kinetics and persistence in mononucleated and binucleated human peripheral lymphocytes following short term and continuous in-vitro exposure to vincristine-sulfate (2068782) (VS) and ethylene-dibromide (106934) (EDB) were examined. The short term exposure lasted 4 hours. VS exposure increased the incidence of micronucleus in both mononucleated and binucleated cells. Continuous VS treatment produced comparatively higher frequencies of micronucleated cells than those treated for the short term exposure. Both mononucleated and binucleated cells demonstrated the highest micronuclei frequencies at 24 hours after chemical treatment. Even in the cells harvested at 144 hours, the micronucleus frequencies remained significantly higher. Many micronucleated cells were induced by VS with multiple micronuclei. A severe decrease was also noted in nuclear division following VS exposure. EDB treatment for 4 hours and continuously demonstrated increased incidences of micronuclei in binucleated cells. In mononucleated cells higher micronucleus frequencies were only observed in continuously treated cultures. Relatively few multiple micronucleated cells were produced by EDB in comparison with VS. EDB did not cause any change in nuclear divisions. The persistence of DNA damage in the cells was evidenced by the high micronucleus frequencies observed at 144 hours harvest following 4 hours of treatment with both EDB and VS. The authors conclude that VS and EDB appear to be genotoxic to human peripheral lymphocytes. The measurement of genotoxicity and cytotoxicity simultaneously at different doses and exposure times may be important in the evaluation of genotoxicants.
NIOSH-Author; Genotoxic-effects; Blood-cells; Chromosome-damage; Chromosome-disorders; Antineoplastic-agents; Brominated-ethylenes; In-vitro-studies
Environmental and Molecular Mutagenesis