Analysis of environmental histamine from agricultural dust.
Siegel-PD; Shahan-TA; Sorenson-WG
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):60-62
A method was developed for the quantitation of histamine (51456) levels from environmental air and bulk samples in the laboratory. The assay was applicable to monitoring of histamine exposure in the workplace. Representative samples of bulk materials to which the agricultural workers can be exposed were collected. Two extraction media, distilled water and perchloric-acid, were evaluated for the extraction of histamine from bulk and filter samples. The most reliable method developed involved perchloric-acid extraction followed by the butanol-acid clean up technique. This method cleaned the extract, removing possible interferences and concentrated the samples. Extracts were alkalinized with sodium- hydroxide and extracted into butanol. After a second extraction the total butanol extract was added to heptane and back extracted into hydrochloric-acid. An aliquot of the hydrochloric-acid was recovered and stored at minus 70 degrees-C until assayed. Hay, chicken coop bedding, rafter dust, wood mulch, silage, and cotton dust demonstrated measurable levels of histamine. Cultures of bacteria and fungi were tested for histamine production. Gram negative bacteria, gram positive bacteria, and fungi were found to produce histamine.
NIOSH-Author; Agricultural-industry; Agricultural-workers; Bacterial-dusts; Airborne-dusts; Respiratory-system-disorders; Plant-dusts; Microorganisms
Dr PD Siegel, Biochemistry Section, NIOSH, 944 Chestnut Ridge Road, MS 211, Morgantown, WV 26505, USA
Scandinavian Journal of Work, Environment and Health