Power analyses and immunoassays for measuring reproductive hormones in urine to assess female reproductive potential in field studies.
Kesner-JS; Krieg-EF Jr.; Knecht-EA; Wright-DM
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):33-36
The use of urinary endocrine biomarkers for field studies of reproductive function in women was discussed. Nonradioisotopic immunoassays for measuring these biomarkers in urine were described. Time resolved fluoroimmunometric assays for urinary luteinizing hormone (LH) and follicle stimulating hormone (FSH) were validated. The results of a urinary endocrine biomarker study indicated that the number of subjects per study group needed to detect a 20% change were 30 to 90 for the timing and magnitude of LH, pregnanediol-3- glucuronide (Pd3G), estrone-3-glucuronide (E13G) and E13G/Pd3G peaks, and the follicular phase length; ten to 30 for lengths of menstrual cycle and luteal phase and for intervals from the LH surge to the Pd3G peak; 120 to 140 for E13G peak magnitude; and over 1000 for intervals from the LH surge to either the E13G peak or the E13G/Pd3G peak.
NIOSH-Author; Biological-monitoring; Urinalysis; Reproductive-hazards; Reproductive-system-disorders; Hormone-activity; Analytical-methods
Dr JS Kesner, National Institute for Occupational Safety and Health, Division of Biomedical and Behavioral Science, Cincinnati, Ohio 45226-1998, USA
Scandinavian Journal of Work, Environment and Health