Measurement of salivary immunoglobulin A as an immunologic biomarker of job stress.
Henningsen-GM; Hurrell-JJ Jr.; Baker-F; Douglas-C; MacKenzie-BA; Robertson-SK; Phipps-FC
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):133-136
As part of a larger investigation of the immunosuppressive effects of job stress, salivary immunoglobulin-A (IgA) levels were measured in nurses. The study group included 40 adult, premenopausal nurses each of whom completed NIOSH job stress questionnaires. Saliva samples were collected weekly for 8 months and blood samples were drawn monthly. Total IgA and titers against lipopolysaccharides from five enteropathogenic Escherichia-coli strains were determined. A modified enzyme linked immunosorbent assay (ELISA) method was used for total and specific IgA antibodies in saliva. The preliminary findings suggested that the ELISA methods were about ten times more sensitive or less variable, more automated, and cheaper than the radioimmunodiffusion kits commonly used for total IgA analysis. The results suggested that the low objective and low subjective stress group consistently had the lowest mean specific salivary IgA titers, but the statistical significance of this finding has not yet been determined.
NIOSH-Author; Immunology; Immunoglobulins; Humans; Health-care-personnel; Job-stress; Nursing; Immune-reaction
Dr JJ Hurrell, Jr, Nat ional Institute for Occupational Safety and Health, 4676 Columbia Parkway (MS·C26), Cincinnati, OH 45226, USA
Scandinavian Journal of Work, Environment and Health